Figure 1.

Effect of different PS-DNA sequences and PS-DNA transfection on PrP^Sc in ScN2a cells. Cells were exposed to different oligonucleotides for 48 h. CpG, PS-DNA that contains the CpG motif as described in Methods; Scr, scrambled sequence of the CpG oligonucleotide; Met, methylated version of CpG oligonucleotide: C, control ScN2a cells without PS-DNA. In all transfected cells, DOTAP was added while the non-transfected cells were incubated with PS-DNA only. Cells were either undigested (-PK) or subjected to PK digestion at 37°C for 1 h (+PK), and all immunoblots were stained with the D13 anti-PrP antibody.