Figure 6.

Lack of enhancement in integration after gene transfer with virosomes and immunovirosomes. HSVECs were transduced with amphotropic MMLV-EGFP either in the presence of polybrene or as virosomes or immunovirosomes (made with anti-CD71 mAb). As a control, immunovirosomes were used containing anti-CD71 and the control amphotropic MMLV-TCR (23). (A) After gene transfer, the cells were cultured for 27 days and on different days (indicated by numbers above the gel) mRNA and genomic DNA was prepared for RT-PCR (top) and Southern blotting (bottom) for detection of EGFP mRNA or DNA, respectively. Also shown are RT-PCR or Southern blots from untransfected cells (U), mock-transduced cells (M), or immediately after exposure to the virus (0). (B) Copies of integrated viral DNA were analyzed. The results are expressed as mean relative copies of integrated DNA ± SD of three independent experiments.