Skip to main content
. Author manuscript; available in PMC: 2008 Apr 30.
Published in final edited form as: Diabetes Res Clin Pract. 2006 Oct 2;76(2):177–186. doi: 10.1016/j.diabres.2006.09.012

Figure 2.

Figure 2

Oligonucleosome ELISA of rat glomeruli epithelial cell lysates, with Roche Cell Death Detection Kit performed according to the manufacturer’s instructions. Rat glomeruli epithlial cells were generously provided by Prof. H. Abboud, UTHSCSA Division of Nephrology. Monolayers were grown to 80–90% confluence in 60 mm dishes. Experimental media was replaced to serum-free (SF) conditions with standard (5 mM) glucose. Cells were harvested with trypsin-EDTA, pelleted, and washed with 1X phosphate-buffered saline. Under serum-free (SF) conditions, exogenous recombinant human IGFBP-3 exposure at 1 mg/mL for 24 h induces apoptosis in the presence of neutralizing IgG to the type 1 IGF receptor (n=8; †p<5% vs. SF control and other p values as shown by two-tailed Student’s t test). As described using mesangial cells in ref. 24, pre-treatment of the rat podocytes with anti-sense IGFBP-3 oligo (but not sense oligo) blocked apoptosis induced by tumor necrosis factor (TNF)-α. As observed with other cell types, IGFBP-3 can induce podocyte apoptosis acting independently of the type 1 IGF receptor.