Table 2.
List of tags present at higher levels in the Otx2−/− than in the WT library
| Tag | WT | KO | KO/WT | P | GenBank match |
|---|---|---|---|---|---|
| ATAAAAAAAA | 0 | 9 | >9 | 0.004 | Peroxisomal PTS2 receptor (U69171) |
| TACGAGCACA | 1 | 9 | 9.0 | 0.018 | EST, highly similar to ubiquinol cytochrome C reductase (AA033187) |
| TTGCCTTATT | 1 | 9 | 9.0 | 0.018 | EST 331499, similar to human IP-30 protein (W13914) |
| TTCCCAGAAA | 1 | 9 | 9.0 | 0.018 | EST, similar to ATP synthase subunit D mitochondrial (D13120) |
| AGCGTGGCCT | 0 | 8 | >8 | 0.008 | AAC-11 inhibitor of apoptosis (U35846) |
| AGCCCCGCCT | 0 | 8 | >8 | 0.008 | Integrin binding protein kinase, ILK (U94479) |
| TCTCTAAATA | 2 | 16 | 8.0 | 0.001 | Cystatin B (U59807) |
| CGCTTGGCAG | 1 | 8 | 8.0 | 0.030 | Δ Proteasome subunit (U13393) |
| TTCTGTGCAG | 1 | 8 | 8.0 | 0.030 | EST J0515B06 (AU0119257) |
| TGACACCCTT | 1 | 8 | 8.0 | 0.030 | EST 633613 (AA184527) |
| TGTTCATACA | 0 | 6 | >6 | 0.018 | ESTs (AI155073; AA433547) |
| TGTAGTCTGC | 0 | 6 | >6 | 0.018 | EST, similar to human argine rich protein (AA217925) |
| CCCCTCCCAC | 0 | 6 | >6 | 0.018 | ESTs (AA871006; AI115602; W64163) |
| TTCTATGCCT | 3 | 19 | 6.3 | 0.001 | EST 493579 (AA087133) |
| TCCTTTGCCC | 0 | 5 | >5 | 0.042 | Zona pellucida (Zp-1) gene, exon 1 (U24227) |
| TGCTGAAGGA | 0 | 5 | >5 | 0.042 | Zinc finger protein Requiem (req) (U10435) |
| TGGGGTAGAT | 0 | 5 | >5 | 0.042 | Calregulin (ERp60) (M92988) |
| TGTGGGAAAA | 0 | 5 | >5 | 0.042 | EST, vanin 3 (AI118382) |
| ATAGCAAAGA | 0 | 5 | >5 | 0.042 | EST, similar to canis familiaris VIP-36 protein (AW229370) |
| AGCACAGCCT | 0 | 5 | >5 | 0.042 | EST 947464 (AI505056) |
| AGTTTGCTCA | 0 | 5 | >5 | 0.042 | Peroxisomal biogenesis factor (Pex11b) (AF093671) |
| CAGGCGGCCA | 0 | 5 | >5 | 0.042 | Catenin α 1 (X59990) |
| GAGTACTTTT | 0 | 5 | >5 | 0.042 | Purine-nucleoside phosphorylase (X56548) |
| CTGAATGACG | 2 | 11 | 5.5 | 0.012 | Nuclear RNA helicase Bat1 (AF118128) |
| GCAGAACCAG | 3 | 16 | 5.3 | 0.003 | A10 mRNA, partial cds (L21027) |
| CTGCTCCTGC | 2 | 10 | 5.0 | 0.025 | Metallothionein 2 (K02236) |
| AGTTTGTCTG | 2 | 10 | 5.0 | 0.025 | EST 1480726 (AI035276) |
| AGCGAAGTGG | 2 | 10 | 5.0 | 0.025 | EST, highly similar to human N-terminal acetyltransferase (AA068754) |
| CAACTCCAAT | 2 | 9 | 4.5 | 0.049 | Calpactin I light chain (p11) (M16465) |
| TTTTGCACAG | 2 | 9 | 4.5 | 0.049 | ESTs 765113; 947489 (J0528F02) |
| CTGAGAACTT | 2 | 9 | 4.5 | 0.049 | β-Globin gene (AA960070) |
| GGAATGCGGG | 2 | 9 | 4.5 | 0.049 | EST 1248132 (AA960070) |
| GTGACTGGGT | 5 | 21 | 4.2 | 0.001 | Mitochondrial cytochrome c oxidase subunit IV (COX IV) (M37829) |
| TTGTTGGAGG | 3 | 11 | 3.7 | 0.033 | EST (W29669) |
| TTAAATAAAA | 3 | 11 | 3.7 | 0.033 | Sid393p (AB025049) |
| GTGTTGTTTA | 5 | 14 | 2.8 | 0.037 | cdc2 mRNA for CDC2 kinase (X16461) |
| CTGTGTGTGG | 7 | 18 | 2.6 | 0.033 | Prostatic secretory glycoprotein (p12) (X06342) |
| TGAAAATTGG | 9 | 23 | 2.6 | 0.015 | COX7cl mRNA for cytochrome c oxidase VIIc (X52940) |
| GACAAGGCCA | 28 | 67 | 2.4 | 0.000 | Apolipoprotein A–I (X64262 S37) |
| ATTTCAAGAA | 9 | 21 | 2.3 | 0.022 | EST 1450276 (AI035675) |
| CACGTTGTCA | 8 | 18 | 2.3 | 0.050 | Embigin precursor (J03535) |
| AATAAACCGT | 8 | 18 | 2.3 | 0.050 | Adenine phosphoribosyltransferase (APRT) (M11310) |
| CAAAAAAAAA | 11 | 24 | 2.2 | 0.025 | EST 863707 (AA512428) |
| TGGGGAAAGG | 13 | 28 | 2.2 | 0.021 | Ribosomal protein L35a (Y16430) |
| TTTGCCGGCA | 27 | 58 | 2.1 | 0.001 | Ubiquitin (X51703) |
| AATAAAGTGG | 16 | 33 | 2.1 | 0.016 | Cyclophilin (M60456) |
| AATGCCCTCA | 65 | 130 | 2.0 | 0.000 | Acidic ribosomal phosphoprotein P1 (U29402) |
| GAGAGGGCAA | 84 | 162 | 1.9 | 0.000 | Ribosomal protein S12 (X15962) |
| TGAAATGAAC | 23 | 44 | 1.9 | 0.010 | EST 475993 (AA049909) |
| GTTCCAAAGC | 24 | 45 | 1.9 | 0.011 | Hexokinase 1 (J05277) |
| CGTATTACCT | 27 | 49 | 1.8 | 0.010 | REX-3 (AP051347) |
| CCGCGAGGCC | 42 | 75 | 1.8 | 0.003 | Ribosomal protein S26 (RPS26) (U67770) |
| TTCCAGGCCC | 20 | 35 | 1.8 | 0.029 | Apolipoprotein E (M12414) |
| AAAGCCCGGA | 79 | 138 | 1.7 | 0.001 | Ribosomal protein S8 (X73829) |
| GCATTGCCAA | 62 | 107 | 1.7 | 0.001 | J1 protein, yeast ribosomal protein L3 homologue (Y00225) |
| AGCAAGCAGG | 45 | 77 | 1.7 | 0.003 | Cytoplasmic beta-actin (MI2481) |
| CTCCTTGTCA | 23 | 38 | 1.7 | 0.048 | Phosphatidylethanolamine binding protein (U43206) |
| AGAAAAAAAA | 36 | 57 | 1.6 | 0.027 | Skeletal muscle chloride channel (X62897) |
| AAACAACCCA | 37 | 56 | 1.5 | 0.045 | Mitochondrial gene for cytochrome b (X57779) |
| AAGCGGGCTT | 51 | 76 | 1.5 | 0.020 | EST, highly similar to human ribosomal 60S protein L34 (AA574826) |
| GGGGTTTACC | 76 | 110 | 1.4 | 0.012 | Ribosomal protein S16 (M11408) |
| AATGATGAGG | 79 | 105 | 1.3 | 0.040 | Heat shock protein 70 cognate (M19141) |
Each tag is given its sequence, its abundance in both libraries (WT and KO), the ratio value (KO/WT), the P value calculated by Monte–Carlo simulations, and its GenBank match. Only tags displaying GenBank matches are shown. The complete list of tags obtained in WT and Otx2−/− will be available at http://www.dsv.cea.fr/thema/get/sade.html.