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. 2007 Jun 27;2(6):e578. doi: 10.1371/journal.pone.0000578

Table 2. Markers used for genotyping of Da(1)-12×Ei-2 RILs.

Marker Chr BAC/P1 Primer 1 (5′→3′) Primer 2 (5′→3′) Gel pattern
C1P12 1 F20B24 CTGGAAGTCCATACCATGAG GTTCGTCGTTCGTGGTATTG D>E
F20D23_2 1 F20D23 CCGTCACACCATTCACAATC CCAACCCCTTATATATCGTTC D>E
MSAT1.7 a 1 F12K8 GCTTTTATCAGCTCAAACAT ACTCTTACGTTTGGAGTTCA D>E
NGA248 a 1 F3H9 TCTGTATCTCGGTGAATTCTCC TACCGAACCAAAACACAAAGG D<E
F1K23 1 F1K23 GAACCAATAAGGAGGCTCAAC CCATACGGAGAAACCTTCTTC D>E
F1K23_2 1 F1K23 CAATTTCGAGTTTCGGATTTTC CTTCACATCAATGCTTGTAATAG D<E
MSAT1.4 a 1 F28L22 CTAAACTAGAACCAGGGGTAA ACAAAAATCGTGGTGATAATA D<E
T27K12 a 1 F7F22 GGAGGCTATACGAATCTTGACA GGACAACGTCTCAAACGGTT D>E
B14 1 F11F12 CCATTCTCGTCGTGTTATAAG GAAATGTTAAGGCCAAAATACAG D<E
B12 1 T18A20 CAACTCGTTATAACAGGTTTTAC CCAAATACTAAAGAGGGAATTG D<E
F5I14 a 1 F5I14 CTGCCTGAAATTGTCGAAAC GGCATCACAGTTCTGATTCC D>E
MSAT1.12 a 1 T26J14 TTAGAGATTCGCCAACCTC CGTGTGCCCAACCA D>E
MSAT1.13 a 1 F24J5 GTCAAACCAGTTCAATCA CAACCACCAGGCTC D<E
MSAT1.1 a 1 F20P5 ATACGATAAGATTTATTAGCA CCCATGCTCTTTTTGTGAAA D>E
MSAT1.2 a 1 F22K20 TTGAGTGGTGCCGCTTG ATATCTCCATCGCTGCAACC D>E
MSAT2.38 a 2 F18P14 TGTAACGCTAATTTAATTGG CGCTCTTTCGCTCTG D>E
MSAT2.18 a 2 T4E14 TAGTCTCTTTTGGTGCGCATA AGCCTCTCCAAGCTTAGGTCT D>E
MSAT2.28 a 2 T26I20 AATAGAAATGGAGTTCGACG TGAACTTGTTGTGAGCTTTG D<E
MSAT2.11 a 2 F19F24 GATTTAAAAGTCCGACCTA CCAAAGAGTTGTGCAA D>E
T20K24 2 T20K24 CAATATTCGTGGGAGTTAGTC GCTGTCGAATTACATTTCTTTAC D<E
F3K23 2 F3K23 CTCGCAGCGTCTGCAAATTC GAAGCGGAAGATGGAGAGAC D>E
MSAT2.31 a 2 T22F11 GCTCCTCTTTGCCGCTAG GCGATTTCATCTTGTGCATC D>E
MSAT2.37 a 2 T19L18 GGTTGTTTCATCGAAAGCA CATGGTCTCGCTGGTGTAT D<E
C22 2 T26B15 CTTGGCAACTTCATTCAATTTC GAAAGTAGAGAAGCATTTAGAC D<E
MSAT2.4 a 2 T26B15 TGGGTTTTTGTGGGTC GTATTATTGTGCTGCCTTTT D<E
A21 2 T1J8 CCATCTAAACTGCTTACGATG GTGACCCATTCTTCTCTTTTC D<E
MSAT2.22 a 2 F17A22 CGATCCAATCGGTCTCTCT TGGTAACATCCCGAACTTC D<E
C3P41 3 F9F8 GGTCGTATCCTCTTATCGAAC CTTGTGAGTGGTCTTATGAAAG D<E
3FM08 3 K20I9 GGTTCGTATCCAAAAACCAAG CCATCATTGGAGCAAGAGAC D>E
C3P43 3 MRC8 CAATGTTGGCTTGGAAATAATG CATTGCCGGTAAAAATGTTTTTC D>E
3FM09 3 MAL21 CTAATTACTATGGCGGAGAATTC CTAAAGAAATCTGCGGTCTTC D>E
3FM10 3 MSD21 CATTACTTCACTGTTGCTTTAC GACAGGTTATGGCTTGTTAATC D>E
MSAT3.19 a 3 K7M2 TAATTCGATCCAATTGACAT TGGCTTGGCACAAAC D<E
3FM12 3 K24A2 TAGGGAAGCATTTGTCTTGAG TGCTTAAAGTGACGGTAAAATG D: 1 band E: 2 bands
MSAT3.8 a 3 K5K13 ATGTTAAAAACCCGTGTTGG TTTAACCTTATCCGGGAAAG D>E
MSAT3.32 a 3 MXO21 GCACTTGCAGCTTAACTT CGTGACTGTCAAACCG D>E
C32 3 T15B3 GAAGAGGATGAACAAAGATAAG CAAATCTGCCTCCTCCATAAG D<E
MSAT3.28 a 3 T26I12 TACAAGTCATAATAGAGGC GGGTTTAGCATTTAGC D<E
T5J8_2 b 4 T5J8 CGATCATCGGTGTTCACCTT GAAAATAAATCGTCATATGGTGTACTG D>E
T5J8_1 b 4 T5J8 GCCAAGACGCAGAAGAAGAG TCTCATTATTCCCCACAATGC D<E
F4C21 4 F4C21 GCGCTTCATCTAGTTACGCTTT CCCGGACTGAACCAAACTAA D>E
NGA8 a 4 T32A17 GAGGGCAAATCTTTATTTCGG TGGCTTTCGTTTATAAACATCC D<E
F28M11 4 F28M11 CACCATATTGGCCTCAAATTG CAAAAACCCGTTCCACCAAAC D<E
MSAT4.25 a 4 F25E4 GAATGGTTGTTGATAGTTGA AAATTTCAGGAGGTGATAGA D<E
F25G13 4 F25G13 GCCAGGTTCTTTTCATTTCTC GGGCGTTTAATTTGCATTCTTC D<E
MSAT4.35 a 4 F25G13 CCCATGTCTCCGATGA GGCGTTTAATTTGCATTCT D<E
T6G15 4 T6G15 GTAGCCAGAGATGGAAGTTAC GGGTCCTTACTGAGGCTTTG D>E
MSAT4.18 a 4 T12H17 TGTAAATATCGGCTTCTAAG CTGAAACAAATCGCATTA D<E
T27E11 4 T27E11 GTGATTCCCGTCTGCTAAAC CCTCCTTCAGCATCATAGTG D>E
F25O24 4 F25O24 CAATGTATTTGGATGTGTTTGTTC GGATGGTAACACGGCTAAAC D<E
FM12 4 T16L4 GAAGCCCCTATGAGATGGTC GTGAGGGAGTTAGGTAGCAAC D<E
FM14 4 F9N11 TCAAGGGAGACTTGGAGAAC GGGATCGTTATGCACTTGTTTG D<E
FM17 4 F9N11 CTCCCTCTTCGGAGAAATTC CATCTCTTATAGGCCTCTCTC D>E
4FM05 4 F17I23 CTAACAGATTTGGTGAATAACAAG TCATTTGATGTGCCAGTAAATC D>E
FM21 4 T10C21 CTCAAGCGGTGGAAATTGGAG GTAAAGAATGTCCAGGGCAG D<E
MSAT4.14 a 4 F8F16 GACCGTTTCTAGTGCTCACA ACGGAATAAGCGGAGGA D<E
FM24 4 F3L17 GAGCATCCGCGTAGGTTAAG CACAGAGAGACTCAAAAATACTG D<E
FM34 4 F11C18 TGGTCTCTCAACTCCAACAC CATTGAGATTTGAGCCAAACAG D<E
MSAT4.11 a 4 F10N7 AAAAATCCGGTAGAGCATCC CCAATTCCGAGCCAGTAA D<E
F10N7 4 F10N7 GTTGCTCGAAACCTCTCAATC GCCTCACCGATACGTTTCTG D<E
4FM01 4 F8B4 AGTAGATACAATGCGTTGACC GGAGCGTTAATAGTGTGTATG D<E
FM29 4 L23H3 GTCCAGGTTGCTGAAGAGAAG GTATTGTTTGGTTGGTATGAGC D<E
MSAT4.9 a 4 F4D11 GAAATCAACGGCTGAG AAGTAATTAAGACGCTGAGA D<E
F4B14 4 F4B14 CGTCGTTTATTTCACCACCAC GGTACAAAGATGGGTTAAACTG D<E
F6G17 4 F6G17 GACACGCAAACAAAGTAAAAGTC GATGGTGACATAGACCCAATG D>E
MSAT4.33 a 4 F6G17 TTCTTTGACACGCAAACA TGGTGACATAGACCCAATG D>E
MSAT4.21 a 4 F19F18 TTATGCTATGGCTGTTTGGT CGAAATCTGTTCTTGCATTC D>E
MSAT4.30 a 4 F20D10 AGAGCACTCACCGTTCAT TGTGTTCGTGGATTTACC D<E
MSAT4.31 a 4 T5J17 AGGGATATGGATTGAGA GCCGTATAACTATTGGTT D<E
NGA249 a 5 MAH20 TACCGTCAATTTCATCGCC GGATCCCTAACTGTAAAATCCC D>E
NGA151 a 5 F18022 GTTTTGGGAAGTTTTGCTGG CAGTCTAAAAGCGAGAGTATGATG D<E
NGA139 a 5 K18P6 GGTTTCGTTTCACTATCCAGG AGAGCTACCAGATCCGATGG D>E
C5P71 5 T26D3 GACGATGGTGGAGTGATAAG CTTTGACCTCAAACTTAAGTAG D<E
MSAT5.25 a 5 MOK9 GCTTAATTTGGGTTAAAT GCACGCAAGTGACT D<E
MSAT5.22 a 5 MWP19 AGAACAAGTTAGGTGGCT GGGACAAGAATGGAGT D<E
C5P81 5 MFO20 GTCAAAGAGTTACTCCGTTAC CGAGACAAGAGCATGTTATATG D<E
MIO24 5 MIO24 GTACAATAATTTAGAGAGTATTTTG CTAGCTCAACTTACTGCTTAATG D<E
MNC6 5 MNC6 GTTTGGGTCCAATGATAAAATC GCCTATTGGGCTGAGTTTTC D>E
MMN10 5 MMN10 CAGTGTCGGCTAATTTCGAC CAGTCGACATTTCAAAGGTTC D<E
5FM10 5 MFB13 GATTTGACGACTGATTACATAAC GCTTGAAATTTGTGTGTATTGTC D>E
5FM09 5 MPA24 CAATTTCTTGTTATCTGCTTATG CCATTGCCATATGTTTCCCTC D<E
b

: Markers are from [14]

Bold-typed markers were used for QTL mapping.