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. 2007 Jun 27;2(6):e571. doi: 10.1371/journal.pone.0000571

Figure 4. Gluc-YFP fusion to visualize secretory pathway in real-time.

Figure 4

(A) Schematic representation of the Gluc-YFP fusion cloned in the lentivirus vector. (B) Uninfected 293T cells or cells infected with a lentivirus vector expressing Gluc-YFP fusion were lysed and analyzed by western blotting with anti-Gluc antibody. (C) Cells expressing Gluc-YFP fusion were treated with BFA or nocodazole and their conditioned medium were assayed for Gluc activity 24 hrs later. *p≤0.01 as predicted by student T-test. (D) Fluorescence microscopy of a single live cell expressing Gluc-YFP and either untreated or treated with BFA showing that this fusion is trapped in the ER upon BFA treatment. Scale bar, 10 µm.