Figure 1.
(A) Design of Modified EKLF Constructs. Transactivation domain of EKLF is replaced by repressor domains, Engrailed and Sin 3/HID. (B) Transcriptional activities of EKLF repressor proteins on pCAC-tkCAT. The pCAC-tkCAT reporter was cotransfected with vector alone, full length EKLF or the repressor constructs into CV1 cells. CAT activity in extracts was normalized to cotransfected growth hormone. The average of 3 experiments is shown.