FIG. 4.

Rarefaction curves generated for 16S rRNA gene libraries from VRP5 and VRP9 sediment samples. Error bars indicate 95% confidence intervals. Clones were grouped into phylotypes based on DNA sequence analysis, with members of phylotypes showing ≥97% sequence identity and the same RFLP patterns. Because of the omission of the chimeric clones, the total numbers of clones considered for the rarefaction analysis were 100, 100, 97, 95, 84, and 100 for the 5V, 5F, 5C, 5S, 9V, and 9S libraries, respectively.