TABLE 1.
Loss of DNA in the extraction procedure
| Treatment and DNA concn (ng/μl) in PCR mixture | Amt (ng) of DNA entering KingFisher | Elution vol (μl), KingFisher |
CT valuea
|
|
|---|---|---|---|---|
| Salmonella/FAM | IAC/HEX | |||
| Direct PCR | ||||
| 0.1 | 20.5 | 30.6 | ||
| 0.1 | 20.3 | 31.4 | ||
| DNA extraction | ||||
| 0.1b | 8 | 80 | 25.6 | 30.1 |
| 0.1b | 8 | 80 | 25.5 | 30.3 |
| Direct PCR | ||||
| 0.05 | 20.6 | 29.8 | ||
| 0.05 | 20.0 | 30.2 | ||
| DNA extraction | ||||
| 0.05b | 4 | 80 | 25.0 | 30.1 |
| 0.05b | 4 | 80 | 25.5 | 30.1 |
| Direct PCR | ||||
| 0.01 | 23.8 | 30.3 | ||
| 0.01 | 23.0 | 31.0 | ||
| DNA extraction | ||||
| 0.01b | 0.8 | 80 | 28.7 | 29.8 |
| 0.01b | 0.8 | 80 | 27.6 | 29.7 |
| Direct PCR | ||||
| 0.005 | 22.7 | 30.3 | ||
| 0.005 | 22.3 | 30.4 | ||
| DNA extraction | ||||
| 0.005b | 0.4 | 80 | 26.9 | 29.9 |
| 0.005b | 0.4 | 80 | 27.0 | 30.2 |
a
CT values obtained in PCR from samples with similar DNA concentrations subjected directly to PCR and following DNA extraction.
b
Estimated DNA concentration if the extraction procedure was 100% efficient.