TABLE 2.

C_T values obtained in PCR comparing duplicate samples with similar DNA concentrations extracted with 60, 75, or 90 μl of paramagnetic particles per sample

DNA concn (ng/μl)	Vol of paramagnetic particles (μl/sample)	CT value
		Salmonella/FAM		IAC/HEX
0.1	60	22.1	22.2	27.0	27.0
0.1	60	22.2	22.3	26.9	26.8
0.1	60	22.7	22.8	27.1	26.6
0.1	75	21.8	21.9	26.9	26.8
0.1	75	22.7	22.5	26.6	26.5
0.1	75	21.9	21.9	26.9	26.6
0.1	90	21.9	21.6	26.9	26.7
0.1	90	21.8	21.9	26.6	26.3
0.1	90	21.8	21.8	26.5	26.4
0.05	60	24.5	24.6	26.6	27.1
0.05	60	23.8	23.7	26.8	27.1
0.05	60	24.1	24.3	26.5	26.9
0.05	75	23.6	23.5	27.3	26.6
0.05	75	23.1	23.1	26.7	26.9
0.05	75	24.5	24.4	26.8	26.5
0.05	90	23.9	23.9	26.7	26.8
0.05	90	23.3	23.4	26.8	27.2
0.05	90	23.2	23.4	27.2	26.5
0.01	60	26.9	26.9	27.1	26.8
0.01	60	26.7	26.3	26.4	26.6
0.01	60	26.9	27.6	27.0	27.4
0.01	75	24.8	24.9	26.8	26.7
0.01	75	26.3	26.8	26.8	27.2
0.01	75	25.0	24.9	26.8	27.0
0.01	90	24.8	25.2	26.5	26.7
0.01	90	25.7	25.7	27.1	26.5
0.01	90	24.7	24.9	26.9	27.2
0.005	60	26.7	26.6	26.7	26.7
0.005	60	27.2	27.4	27.0	26.8
0.005	60	27.3	27.3	26.9	27.1
0.005	75	26.6	26.6	27.0	26.5
0.005	75	26.0	26.3	26.7	26.8
0.005	75	27.8	27.6	27.1	27.2
0.005	90	26.2	26.2	27.4	26.8
0.005	90	25.8	25.6	26.9	26.8
0.005	90	26.3	26.3	27.0	26.8