TABLE 3.
Results obtained by analyzing artificially inoculated minced pork meat and poultry samples for the presence of Salmonella by the PCR methoda
| Sample | No. of CFU inoculated (organism)b | PCR (CT value)
|
|
|---|---|---|---|
| FAMc | HEXd | ||
| Minced pork meat | |||
| 1 | 1-10 (st) | 28.7 | 33.3 |
| 2 | 1-10 (st) | 37.7 | 32.9 |
| 3 | 1-10 (st) | NAe | 32.6 |
| 4 | 1-10 (st) | 36.4 | 32.4 |
| 5 | 1-10 (st) | 33.7 | 32.1 |
| 6 | 1-10 (st) | 33.6 | 32.3 |
| 7 | 1-10 (st) | 37.8 | 32.4 |
| 8 | 1-10 (st) | 33.7 | 32.5 |
| 9 | 1-10 (st) | 35.2 | 32.1 |
| 10 | 1-10 (sl) | 24.8 | 34.9 |
| 11 | 1-10 (sl) | 23.7 | 34.8 |
| 12 | 1-10 (sl) | 23.3 | 34.5 |
| 13 | 1-10 (sl) | 25.5 | 33.5 |
| 14 | 1-10 (sl) | 25.8 | 25.7 |
| 15 | 1-10 (sl) | 24.5 | 34.1 |
| 16 | 1-10 (sl) | 27.1 | 34.7 |
| 17 | 1-10 (sl) | 29.9 | 33.9 |
| 18 | 1-10 (sl) | 27.2 | 34.6 |
| 19 | 1-10 (sl) | 24.7 | 34.9 |
| 20 | 10-100 (sl) | 24.0 | 31.4 |
| 21 | 10-100 (sl) | 24.2 | 32.1 |
| 22 | 10-100 (sl) | 21.1 | 32.6 |
| 23 | 10-100 (sl) | 23.6 | 31.5 |
| 24 | 10-100 (sl) | 23.9 | 31.0 |
| 25 | 10-100 (sl) | 25.6 | 31.2 |
| 26 | 10-100 (sl) | 24.2 | 31.1 |
| 27 | 10-100 (sl) | 25.1 | 31.5 |
| 28 | 10-100 (sl) | 26.6 | 31.6 |
| 29 | 10-100 (sl) | 22.1 | 32.0 |
| 30 | 10-100 (sl) | 26.2 | 31.8 |
| 31 | 10-100 (sl) | 25.5 | 31.3 |
| 32 | 10-100 (sl) | 26.7 | NA |
| 33 | 10-100 (sl) | 24.9 | 32.5 |
| Poultry skin | |||
| 34 | 1-10 (se) | 28.0 | 28.2 |
| 35 | 1-10 (se) | 29.2 | 28.7 |
| 36 | 1-10 (se) | 31.5 | 27.3 |
| 37 | 1-10 (se) | 29.6 | 27.6 |
| 38 | 1-10 (se) | 33.2 | 27.5 |
| 39 | 1-10 (se) | 28.4 | 28.0 |
| 40 | 1-10 (se) | 30.6 | 27.2 |
| 41 | 1-10 (se) | 29.0 | 28.5 |
| 42 | 1-10 (se) | 29.3 | 28.4 |
| 43 | 1-10 (se) | 29.4 | 28.9 |
| 44 | 1-10 (se) | 28.3 | 28.1 |
| 45 | 1-10 (se) | 29.7 | 28.6 |
| 46 | 1-10 (se) | 28.2 | 27.3 |
| 47 | 1-10 (se) | 28.8 | 28.1 |
| 48 | 1-10 (se) | 29.8 | 27.5 |
| 49 | 1-10 (st) | 24.8 | 27.3 |
| 50 | 1-10 (st) | 26.7 | 27.5 |
| 51 | 1-10 (st) | 26.9 | 27.7 |
| 52 | 1-10 (st) | 26.3 | 27.4 |
| 53 | 1-10 (st) | 27.5 | 28.2 |
| 54 | 1-10 (st) | 25.2 | 27.3 |
| 55 | 1-10 (st) | 26.6 | 28.3 |
| 56 | 1-10 (st) | 25.1 | 27.4 |
| 57 | 1-10 (st) | 24.9 | 27.5 |
| 58 | 1-10 (st) | 25.5 | 27.3 |
| 59 | 1-10 (st) | 25.6 | 27.7 |
| 60 | 1-10 (st) | 26.9 | 25.8 |
| 61 | 1-10 (st) | 23.8 | 25.9 |
| 62 | 1-10 (st) | 25.7 | 25.9 |
a
All samples were found positive by the reference culture method.
b
st, S. enterica serovar Typhimurium; sl, S. enterica serovar Livingstone; se, S. enterica serovar Enteritidis.
c
Salmonella target.
d
Internal amplification control.
e
NA, no amplification.