. 2007 Mar 9;73(9):2911–2918. doi: 10.1128/AEM.02176-06

TABLE 1.

Phaeoacremonium isolates used in this study

Phaeoacremonium species	No. of isolates	GenBank accession no.^a	Origin	Yr of isolation
P. parasiticum		U31841	CBS860.73^b^,^c^,^d	1973
			CBS113591^e
	2 (1)^f		Plant nursery in Murcia, Spain	2004
	2 (1)		Plant nursery in Cataluña, Spain	2005
	1 (1)		Cataluña, Spain	2005
P. rubrigenum		AF118139	CBS498.94^c	1994
P. inflatipes		AF197990	CBS391.71^c^,^d	1966
			CBS166.75^e	1974
P. mortoniae		AF295328	CBS211.97^e
			CBS101585^c^,^d	1998
P. viticola		AF118137	CBS113065^e	2001
			CBS101738^c^,^d	1993
	1 (1)		Cataluña, Spain	2005
P. angustius		AF197974 ^g
			CBS114992^c^,^d	1992
			CBS114991^e
P. krajdenii			CBS109479^d	2001
P. venezuelense			CBS651.85^d	1974
P. scolyti			CBS113593^d
			CBS113597^e	1999
P. aleophilum		AF017651	CBS246.91^c^,^d	1996
			CBS110753^e	1998
	1		Toscana, Italy
	16 (1)		Ciudad Real, Spain	2003
	2 (2)		Ciudad Real, Spain	2004
	4		Cataluña, Spain	2005
	2		Plant nursery in Portugal	2004
	5 (1)		Plant nursery in Valencia, Spain	2004
	1		Plant nursery in Murcia, Spain	2004
	2		Plant nursery in Cataluña, Spain	2005

GenBank accession numbers of the ITS sequences used to design the primers and the discriminant restriction enzyme digestion patterns.

CBS isolates are from the Centraalbureau voor Schimmelcultures, Baarn, The Netherlands.

Ex-type strains used to optimize PCR conditions.

Ex-type strains used to test PCR-RFLPs.

CBS isolates used to validate the identification by PCR-RFLP.

The numbers in parentheses are the numbers of field isolates identified by ITS sequences (and β-tubulin for P. viticola) used to validate the identification by PCR-RFLP.

ITS sequence of CBS isolate 249.95. This was the original holotype of P. angustius, which was replaced with CBS114992 when lethally contaminated.