TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant phenotype or genotypea | Source or reference |
|---|---|---|
| Strains | ||
| C. necator | ||
| JMP134 | 2,4,6-TCP+ 2,4-D+; pJP4 | DSMZb |
| JMP134 tcpR | 2,4,6-TCP− Apr Kmr | This study |
| JMP134 tcpX | 2,4,6-TCP− Apr Kmr | This study |
| JMP134 tcpA | 2,4,6-TCP− Apr Kmr | This study |
| JMP134 tcpC | 2,4,6-TCP− Apr Kmr | This study |
| JMP134 tcpY | 2,4,6-TCP+ Apr Kmr | This study |
| JMP134 tcpD | 2,4,6-TCP+ Apr Kmr | This study |
| JMP134 ReutA1582 | 2,4,6-TCP+ Apr Kmr | This study |
| JMP134 tfdFI | 2,4,6-TCP+ Kmr | This study |
| JMP134 tfdFII | 2,4,6-TCP+ Kmr | This study |
| JMP134 ReutB4129 | 2,4,6-TCP+ Apr Kmr | This study |
| JMP134 ReutB4694 | 2,4,6-TCP+ Apr Kmr | This study |
| JMP134 ReutC5982 | 2,4,6-TCP+ Apr Kmr | This study |
| E. coli BW25113 | pKD46; red recombinase | 6 |
| Plasmids | ||
| pRK600 | Cmr | 12 |
| pCR2.1 TOPO-TA | Apr Kmr | Invitrogen |
| pMLS7 | PS7 promoter; Tpr | 19 |
| pS7X | tcpX; pMLS7 derivative | This study |
| pS7XA | tcpX-tcpA; pMLS7 derivative | This study |
| pS7A | tcpA; pMLS7 derivative | This study |
| pS7AB | tcpA-tcpB; pMLS7 derivative | This study |
| pS7C | tcpC; pMLS7 derivative | This study |
| pS7D | tcpD; pMLS7 derivative | This study |
| pCM132 | lacZ promoter-probe vector; Kmr | 22 |
| pPRTCP | tcpR-PtcpX-tcpX′; pCM132 derivative | This study |
| pPTCP | PtcpX-tcpX′; pCM132 derivative | This study |
| pJP4-tfdFI mutant | tfdFI Kmr | This study |
| pJP4-tfdFII mutant | tfdFII Kmr | This study |
a
2,4,6-TCP+ and 2,4-D+, able to grow in 2,4,6-TCP or 2,4-D, respectively; tcp, catabolic genes involved in 2,4,6-TCP degradation; FAD, FAD synthase; Apr, ampicillin resistance; Cmr, chloramphenicol resistance; Tpr, trimethoprim resistance; Kmr, kanamycin resistance.
b
DSMZ, Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Braunschweig, Germany.