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. 2007 Feb 23;73(9):2769–2776. doi: 10.1128/AEM.02584-06

TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmid Relevant phenotype or genotypea Source or reference
Strains
    C. necator
        JMP134 2,4,6-TCP+ 2,4-D+; pJP4 DSMZb
        JMP134 tcpR 2,4,6-TCP Apr Kmr This study
        JMP134 tcpX 2,4,6-TCP Apr Kmr This study
        JMP134 tcpA 2,4,6-TCP Apr Kmr This study
        JMP134 tcpC 2,4,6-TCP Apr Kmr This study
        JMP134 tcpY 2,4,6-TCP+ Apr Kmr This study
        JMP134 tcpD 2,4,6-TCP+ Apr Kmr This study
        JMP134 ReutA1582 2,4,6-TCP+ Apr Kmr This study
        JMP134 tfdFI 2,4,6-TCP+ Kmr This study
        JMP134 tfdFII 2,4,6-TCP+ Kmr This study
        JMP134 ReutB4129 2,4,6-TCP+ Apr Kmr This study
        JMP134 ReutB4694 2,4,6-TCP+ Apr Kmr This study
        JMP134 ReutC5982 2,4,6-TCP+ Apr Kmr This study
    E. coli BW25113 pKD46; red recombinase 6
Plasmids
    pRK600 Cmr 12
    pCR2.1 TOPO-TA Apr Kmr Invitrogen
    pMLS7 PS7 promoter; Tpr 19
    pS7X tcpX; pMLS7 derivative This study
    pS7XA tcpX-tcpA; pMLS7 derivative This study
    pS7A tcpA; pMLS7 derivative This study
    pS7AB tcpA-tcpB; pMLS7 derivative This study
    pS7C tcpC; pMLS7 derivative This study
    pS7D tcpD; pMLS7 derivative This study
    pCM132 lacZ promoter-probe vector; Kmr 22
    pPRTCP tcpR-PtcpX-tcpX′; pCM132 derivative This study
    pPTCP PtcpX-tcpX′; pCM132 derivative This study
    pJP4-tfdFI mutant tfdFI Kmr This study
    pJP4-tfdFII mutant tfdFII Kmr This study
a

2,4,6-TCP+ and 2,4-D+, able to grow in 2,4,6-TCP or 2,4-D, respectively; tcp, catabolic genes involved in 2,4,6-TCP degradation; FAD, FAD synthase; Apr, ampicillin resistance; Cmr, chloramphenicol resistance; Tpr, trimethoprim resistance; Kmr, kanamycin resistance.

b

DSMZ, Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Braunschweig, Germany.