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Effects of temperature (A) and pH (B) on the activity of the purified recombinant AgaV. The effect of temperature on the enzyme activity was determined under standard assay conditions at temperatures ranging between 10 and 90°C. The effect of pH on the enzyme activity was determined under standard assay conditions but in three different buffers: 50 mM citric acid-sodium phosphate (▪), 50 mM KH2PO4-NaOH (▴), and 50 mM glycine-NaOH (⧫).
