TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid(s) | Relevant characteristic(s)a | Source |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | Aps Kns; host strain for general cloning | Clontech (United States) |
| BL21(DE3) | Aps Kns; host strain for expression of recombinant agaV | Novagen (United States) |
| Vibrio spp. | ||
| V134 | Marine isolate, carries agaV | This study |
| T4 | Fish pathogen | This study |
| S. iniae | ||
| G26 | Fish pathogen | This study |
| Plasmids | ||
| pACYC177 | Apr Knr; cloning vector | New England Biolabs (China) |
| pBR322 | Apr; cloning vector | TaKaRa (China) |
| pBK | Knr; pBR322 derivative | This study |
| pBS-T | Apr; TA cloning vector | Tiangen, China |
| pBU | Knr; pBR322 derivative, carries signal sequence-lacking agaV | This study |
| pBUS series | pBU derivatives, carry secretion signal sequences derived from T4 | This study |
| pBUG series | pBU derivatives, carry secretion signal sequences derived from G26 | This study |
| pET25b | Apr; general expression vector | Novagen (United States) |
| pET28a | Knr; general expression vector | Novagen (United States) |
| pET258 | Knr; pET28 derivative | This study |
| pETUA | pET258 carrying agaV structural gene | This study |
| pUALS | Apr; carries signal sequence-lacking agaV | This study |
| pUA series | pBR322 derivatives, carry agaV | This study |
a
Ap, ampicillin; Kn, kanamycin.