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Primers used in this study
| Primer | Sequence (5′→3′)a |
|---|---|
| KnF2 | ATGGATCCACGGTTGATGAGAGC(BamHI) |
| KnR3 | GCCGTCGACCAATTAACCAATTC(HincII) |
| UAF5 | ATGGATCCGAAGACTGGCGAGAAATC(BamHI) |
| UAR3 | CACTCGAGTTGTATAAATTTCAATCGC(XhoI) |
| UAF3 | GAGCATATGAAATCCATAATTAAAAC(NdeI) |
| ApF2 | CAACAGCTGACGAAAGGGCCTCGT |
| ApR1 | TACCAGCTGACAGTTACCAATGC |
| 8F | AGAGTTTGATCCTGGCTCAG |
| 1492R | GGTTACCTTGTTACGACTT |
Underlined nucleotides are restriction sites of the enzymes indicated in parentheses at the ends of the sequences.
