TABLE 3.
Effects of different cations on the activity of the purified recombinant AgaVa
| Cation | Relative activity (%) of AgaV at cation concn of:
|
||
|---|---|---|---|
| 1 mM | 10 mM | 100 mM | |
| Na+ | 170 | 206 | 340 |
| K+ | 151 | 205 | 252 |
| Ca2+ | 108 | 107 | 1.2 |
| Co2+ | 104 | 0 | 0 |
| Fe2+ | 62 | 0 | 0 |
| Mg2+ | 241 | 137 | 45 |
| Mn2+ | 0.64 | 0 | 0 |
The activities are expressed as percentages of the enzyme activity measured in 50 mM sodium phosphate buffer (pH 7.0) alone.