TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Characteristicsa | Reference or source |
|---|---|---|
| E. coli strains | ||
| DH5α | F−endA1 relA φ80 lacZΔM15 hsdR17 supE44 thi-1 recA1 gyrA96 Nalr | 24 |
| KAM3 | ΔacrA strain derived from E. coli TG1 | 40 |
| KAM32 | ΔacrA ΔyghE strain derived from E. coli TG1 | 30 |
| R. solanacearum strains | ||
| K60 | Wild-type race 1 biovar 1 tomato isolate | 33 |
| KDF | K60 dinF::Gmr | This study |
| K1833 | K60 acrA::Gmr | This study |
| K60-phcA | K60 phcA::Ω, Smr | 1 |
| K999 | K60 rpoS Kmr | 9 |
| K200 | K60 hrpB::Gmr | 51 |
| Plasmids | ||
| pBluescript SK(−) | Apr | Stratagene |
| pLAFR3 | Tcr, replicates in R. solanacearum | 61 |
| PLAFacrA | acrA-containing cosmid (7-43), Tcr | This study |
| PLAFdinF | dinF-containing cosmid (6-43), Tcr | This study |
| pSTBlue-1 | AT cloning vector, Apr Kmr | Novagen, Inc. |
| pSTacrA SOE | SOE PCR product with introduced BglII restriction site | This study |
| pSTacrA::Gm | acrA ORF disrupted by Gmr cassette | This study |
| pSTdinF::Gm | dinF ORF disrupted by Gmr cassette | This study |
| pUCGm | Gentamicin resistance cassette (aacC1) | 60 |
| pSTacrA | acrA ORF in pSTBlue-1 | This study |
| pSTdinF | dinF ORF in pSTBlue-1 | This study |
| pBSdinF | dinF ORF in pBluescript | This study |
| pVO155 | Promoter probe plasmid with promoterless uidA gene, Kmr | 44 |
| pDB155 | Promoter probe plasmid with promoterless uidA gene, derived from pVO155, Smr | 9 |
| pVOdinF | dinF promoter and 5′ region (600 bp) in pVO155 | This study |
| pDBdinF | dinF promoter and 5′ region (600 bp) in pDB155 | This study |
| pVOacrA | acrA promoter and 5′ region (1,200 bp) in pVO155 | This study |
| pDBacrA | acrA promoter and 5′ region (1,200 bp) in pDB155 | This study |
| pPilA-FOR | pilA promoter and 5′ region in pVO155 in forward orientation | 9 |
a
Ap, ampicillin; Tc, tetracycline; Gm, gentamicin; Km, kanamycin; Sm, streptomycin; Nal, nalidixic acid.