TABLE 3.
MICs for E. coli strains KAM3 and KAM32 harboring acrA or dinF from R. solanacearum
| Compound | MIC (mg/ml)a
|
||||
|---|---|---|---|---|---|
| KAM3b | KAM3 (pLAFacrA) | KAM3 (pLAFdinF) | KAM32b | KAM32 (pLAFdinF) | |
| Acriflavine | 0.49 | 3.9 | 3.9 | 1.9 | 7.8 |
| Ampicillin | 3.9 | 7.8 | 3.9 | 3.9 | 31.3 |
| Berberine | 31.3 | 62.5 | 125.0 | 15.6 | 31.3 |
| Esculetin | 500 | 500 | 500 | NDc | ND |
| Ethidium bromide | 3.9 | 7.8 | 7.8 | 0.95 | 15.6 |
| Nalidixic acid | 1.9 | 1.9 | 3.9 | ND | ND |
| TPPCl | 3.9 | 3.9 | 3.9 | 15.6 | 31.3 |
a
The MICs are the minimum concentrations required to inhibit bacterial growth. The assays were performed in triplicate to ensure consistent results. The optical densities at 600 nm were normalized to 0.001 at the beginning of the MIC assay. MICs that differ from the MICs for the parent mutant strains are indicated by boldface type.
b
KAM3 and KAM32 with empty plasmid pLAFR3 gave identical results.
c
ND, no data.