TABLE 5.
Relative induction of acrA and dinF in the presence of various compoundsa
| Compoundb | Induction of:
|
|
|---|---|---|
| acrA | dinF | |
| Acriflavine | 2.9 (0.03)c | 1.6 (0.06) |
| Berberine | 1.3 (0.09) | 1.9 (0.02) |
| Esculetin | 1.5 (0.02) | 2.0 (0.03) |
| H2O2 | 3.2 (0.02) | 1.6 (0.17) |
| Mitomycin C | 5.3 (0.07) | 5.1(0.10) |
| Salicylic acid | 1.3 (0.01) | 2.1 (0.03) |
| BMM alone | 1.0 | 1.0 |
a
Relative induction was measured by determining GUS activity from acrA::uidA and dinF::uidA reporter gene fusions. The levels of expression are the levels relative to basal gene expression in minimal medium plus glucose (BMM).
b
Compounds were added at the following sublethal concentrations: acriflavine, 31.25 μg/ml; berberine, 31.25 μg/ml; esculetin, 16.25 μg/ml; H2O2, 5 mM; mitomycin C, 1.0 μg/ml; and salicylic acid, 500 μg/ml.
c
The data are means of three determinations; the values in parentheses are the standard errors.