TABLE 2.
Numbers of viable cells; glucose, ethanol, and nitrogen concentrations; and total RNA and poly(A) signal per 108 cells for each sampling pointa
| Expt | Time (h) | Sampling point | Glucose (g·liter−1) | Nitrogen (mg·liter−1) | Ethanol (%) (vol/vol) | CFU (106)·ml−1 | Total RNA/108 cells (μg) | Poly(A) signal/108 cells |
|---|---|---|---|---|---|---|---|---|
| CF | 24 | 1 | 183.8 ± 0.6 | 178.1 ± 6.4 | 0.6 ± 0.3 | 21.0 ± 9.7 | 48.9 ± 1.7 | 2,530 ± 474 |
| 48 | 2 | 136.1 ± 0.3 | 2.2 ± 2.4 | 3.9 ± 0.7 | 122.5 ± 26.3 | 19.2 ± 1.4 | 1,252 ± 130 | |
| 96 | 3 | 24.7 ± 4.0 | 0.0 ± 0.0 | 9.0 ± 0.7 | 122.5 ± 22.2 | 14.4 ± 3.2 | 1,051 ± 508 | |
| LF | 24 | 4 | 189.1 ± 0.7 | 2.3 ± 0.2 | 0.4 ± 0.1 | 20.3 ± 6.1 | 51.7 ± 2.8 | 2,325 ± 245 |
| 48 | 5 | 171.5 ± 5.7 | 0.0 ± 0.0 | 1.4 ± 0.2 | 69.4 ± 7.2 | 21.2 ± 1.1 | 819 ± 143 | |
| 80 | 6 | 154.1 ± 4.4 | 0.0 ± 0.0 | 2.1 ± 0.1 | 70.3 ± 9.1 | 19.2 ± 1.0 | 978 ± 126 | |
| 96 | 7 | 148.3 ± 4.3 | 0.0 ± 0.0 | 2.5 ± 0.2 | 71.6 ± 6.0 | 18.2 ± 2.5 | 1,207 ± 56 | |
| 144 | 8 | 137.0 ± 3.7 | 0.0 ± 0.0 | 3.2 ± 0.4 | 72.3 ± 4.5 | 15.9 ± 0.2 | 1,264 ± 91 | |
| RF | 80 | 9 | 148.2 ± 1.7 | 136.5 ± 3.9 | 2.2 ± 0.1 | 71.0 ± 4.1 | 22.2 ± 1.7 | 1,264 ± 91 |
| 96 | 10 | 116.6 ± 3.6 | 0.8 ± 1.4 | 4.2 ± 0.4 | 149.6 ± 15.2 | 17.5 ± 4.4 | 987 ± 366 | |
| 144 | 11 | 24.9 ± 10.8 | 0.0 ± 0.0 | 8.9 ± 1.0 | 210.0 ± 33.7 | 22.7 ± 0.8 | 1,856 ± 134 |
a
CF, control fermentation (267 mg of N·liter−1); LF, N-limiting fermentation (66 mg of N·liter−1); RF, refed fermentation. Results are expressed as averages ± standard deviations.