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Effects of mutations in A. niger PhyA on hydrolysis of phytate-phosphorus from soybean meal at concentrations of 250, 500, 750, and 1,000 U/kg of soybean meal at 37°C in 0.2 M citrate buffer for 1 h at pH 5.5 (A) and pH 3.5 (B). Represented are WT (⋄), A58E Q191R (□), A58E Q191R T271R (▵), A58E P65S (×), and A58E P65S Q191R T271R (*). An asterisk indicates a difference (P < 0.05) between each mutant and the WT.
