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Structural prediction of the residual interactions in A. fumigatus phytase (Afp) before and after substitutions at E35, R168, and R248. Green dotted lines represent the hydrogen bond interactions: predicted changes in hydrogen bond interactions caused by the substitution E35A (A); predicted changes in ionic interactions caused by the substitution R168A (B); and predicted changes in ionic interactions caused by the substitution R248A (C).
