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Specific activities and kinetic parameters of WT and selected mutant PhyA phytases at pH 5.5
| PhyA phytase | Sp act (U/mg) (mean ± SD) | Vmax (μM/min ·mg) | Km (μM) |
|---|---|---|---|
| WT PhyA | 80.9 ± 1.1 | 135.1 | 167.5 |
| PhyA(A58E Q191R) | 82.2 ± 1.2 | 128.2 | 154.0 |
| PhyA(A58E Q191R T271R) | 83.8 ± 4.1 | 169.5 | 162.3 |
| PhyA(A58E P65S) | 84.2 ± 3.4 | 109.9 | 130.4 |
| PhyA(A58E P65S Q191R T271R) | 83.2 ± 2.1 | 117.6 | 106.4 |
