TABLE 2.
Identification of flagellin proteins by tryptic digest and nLC-MS/MS analysis
| Strain | Mol mass (kDa)
|
MASCOT score (no. of peptides) | |
|---|---|---|---|
| Predicted | Observed | ||
| Group I | |||
| Type A | |||
| FE9909ACS Alberta | 29.4 | 30.3 | 450 (8) |
| 17A | 30 | 28.0 | 308 (6) |
| Type B | |||
| MRB | 29.5 | 32.1 | 253 (5) |
| FE0507BLP | 29.6 | 28.9 | 213 (3) |
| 30.7 | 174 (2) | ||
| 32.7 | 206 (3) | ||
| Type AB | |||
| FE9504ACG | 29.7 | 28.6 | 363 (6) |
| 30.5 | 265 (5) | ||
| Type F | |||
| Langeland | 29.4 | 29.3 | 133 (3) |
| Group II | |||
| Type B | |||
| 17B | 29.1 | 32.6 | 132 (2) |
| 38.3 | 79 (1) | ||
| 43.6 | 86 (1) | ||
| Kapchunka B3 | 29.1 | 33.0 | 81 (1) |
| Type E | |||
| Bennett | 28.9 | 30.2a | 81 (1) |
| 31.8 | 188 (3) | ||
| 53.0 | 84 (1) | ||
| GA9709EHS | 28.9 | 30.2a | 437 (7) |
| 31.5 | 176 (3) | ||
| 47.7 | 102 (1) | ||
| Type F | |||
| 610F | 29.3 | 33.2 | 82 (1) |
| 38.8 | 70 (1) | ||
| 41.9 | 79 (1) | ||
a
Bands were excised from overloaded SDS-PAGE gels and are not visible in Fig. 2.