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. 2000 Dec 19;97(26):14394–14399. doi: 10.1073/pnas.97.26.14394

Figure 2.

Figure 2

Ski antagonizes BMP signaling in Xenopus explants. (A) Ski blocks BMP2 activity. Explants were cultured until stage 10.5 and analyzed for Xbra expression by RT-PCR. Lanes 1 and 2, whole embryo control and no RT (RT−); lane 3, uninjected explants; lanes 4 and 5, explants expressing AB2; lanes 6–8, explants expressing AB2 with increasing amounts of ski mRNA; lanes 9–11, explants expressing ski mRNA only. (B) Ski fails to neuralize in the presence of high levels of the constitutive active BMPRI (Alk3*). Explants were analyzed at stage 20 for NCAM expression. Lanes 3–5, explants expressing AB2 mRNA only; lane 6–8, explants expressing ski and increasing amounts of Alk3* as indicated. Alk3*, (Q233D)Alk3. (C) Ski blocks the activity of Smad1. Lane 3, uninjected sample; lane 4, Smad1 stimulates the expression of the ventral markers, Xvex-1 and Xhox-3; lanes 5–7, explants expressing Smad1 mRNA in combination with increasing amounts of ski mRNA; Lane 8, ski alone.