TABLE II.
The Effect of IFG and NB-DNJ on the Processing of N-Linked Oligosaccharides From Wild-Type Fibroblasts
| % total radioactivity in each pool | |||
|---|---|---|---|
| Con A I | Con A II | Con A III | |
| untreated: | 26.1 | 16.6 | 57.3 |
| [NB-DNJ] | |||
| 10μM: | 23.8 | 14.6 | 61.6 |
| 100μM: | 21.4 | 15.1 | 63.5 |
| 500μM: | 16.5 | 14.7 | 68.8 |
| [IFG] | |||
| 100μM: | 25.7 | 16.3 | 58.0 |
| 200μM: | 25.6 | 17.9 | 56.5 |
| 500μM: | 24.4 | 17.8 | 57.9 |
Wild-type fibroblasts were treated for 24hr with various concentrations of IFG or NB-DNJ prior to pulse/chase labeling with [2-3H] mannose and glycopeptide preparation. Labeled glycopeptides were fractionated by Con A-sepharose chromatography into three pools representing the different types of N-linked oligosaccharides. A representative analysis is shown. Less than 5% deviation in the pool distribution was observed between independent experiments.