Table 2.
Ferret cloning using different methods for nuclear transfer and somatic donor cell types
Method of nuclear transfer | Donor cell (type) | Nuclear transfers (no. embryos transferred) | Embryo transfers (no. recipients) | 3-week implantation (%) | 3-week fetal development (%) | Birth rate (%) |
---|---|---|---|---|---|---|
Microinjection | Fibroblast | 460 | 15 | 7.2 (25/349) | 3.4 (12/349) | 0 (0/111) |
Cumulus cell | 391 | 15 | 14.2 (44/310) | 4.2 (13/310) | 1.2 (1/81) a | |
Cell Fusion | Fibroblast | 430 | 14 | 5.5 (18/326) | 3.1 (10/326) | 0.96 (1/104) b |
Cumulus cell | 487 | 14 | 4.3 (16/375) | 3.2 (12/375) | 1.8 (2/112) c |
Female clone died hours after birth.
Male clone died 3 days after birth.
Healthy reproductively competent female clones.