Table 5.
Microsatellite genotyping of ferret DNA
| Ferrets and cells | Microsatellite DNA markers
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Mer022 | Mvi87 | G1A | Mvis072 | Mvi232 | Gg4 | Mvis022 | Mer095 | Mvi57 | Mvis075 | Mer049 | |
| Donor cumulus cells (Sable) | 245/245 | 78/78 | 160/162 | 269/269 | 155/155 | 90/90 | 282/284 | 148/150 b | 106/106 | 139/139 | 183/185 |
| Libby a | 245/245 | 78/78 | 160/162 | 269/269 | 155/155 | 90/90 | 282/284 | 148/150 b | 106/106 | 139/139 | 183/185 |
| Lillya | 245/245 | 78/78 | 160/162 | 269/269 | 155/155 | 90/90 | 282/284 | 148/150 b | 106/106 | 139/139 | 183/185 |
| Surrogate mother (albino) | 245/245 | 76/78 | 162/162 | 269/269 | 153/155 | 90/90 | 284/284 | 152/152 b | 106/106 | 139/139 | 185/185 |
| Donor fibroblast cells | 245/245 | 76/78 | 160/162 | 269/271 | 155/155 b | 90/90 | 284/284 | 150/152 | 106/108 | 139/139 | 183/183b |
| Clone c | 245/245 | 76/78 | 160/162 | 269/271 | 155/155 b | 90/90 | 284/284 | 150/152 | 106/108 | 139/139 | 183/183b |
| Surrogate mother | 245/245 | 76/78 | 160/162 | 269/271 | 153/159 b | 90/90 | 284/284 | 152/152 | 104/106 | 139/139 | 185/185b |
AL01–AL02: unrelated albino fibroblasts; SA01–SA10: unrelated sable fibroblasts; ND: not determined; FCC1: ferret cumulus cells unrelated to cloned animals.
Lilly and Libby–Sable coat-color ferrets cloned from cumulus cells (Fig. 1).
Informative markers distinguishing somatic-cell-derived clones from their surrogate mother. Analysis of 13 unrelated DNAs from Albino and Sable coat-color ferrets is shown in Table S1.
Clone–Live-born male ferret cloned from fibroblast cells and died 3 days after birth.