Figure 8. Immuno-detection of cystatin C in the conditioned media of BSC-1 cells expressing recombinant human stefin.
A. Conditioned media from wild type BSC-1 cells (C), infected BSC-1 cells (IC), and transfected BSC-1 cells (T) were subjected to 16% tricine SDS-PAGE, transferred to nitrocellulose membrane, and probed with a polyclonal antiserum to human cystatin C. hCC, 0.05 mg human cystatin C.