Fig. 2.
Increased systemic Aβ after Aβ1–42/CT t.c. immunization of PSAPP mice. For Aβ analysis, blood samples were individually collected from Aβ/CT or CT alone t.c.-immunized PSAPP mice at the time points indicated. (A) Plasma Aβ antibody titers were measured by ELISA. Data are presented as mean ± SD (n = 9) of Aβ antibodies (pg/ml plasma) (∗∗, P < 0.001) and between time points within the Aβ/CT t.c.-immunized group as indicated (##, P < 0.001). (B and C) Plasma Aβ1–40,42 peptides were measured separately by Aβ ELISA. Data are presented as mean ± SD (n = 9) of Aβ1–40 or Aβ1–42 (pg/ml plasma). ∗, P < 0.05; ∗∗, P < 0.001. Arrows indicate each t.c. immunization with respect to the time of blood sample collection.