Table 2.
Secretion of IL-8 and IL-1β by Caco-2 cells after invasion by bacteria treated with preimmune and immune sera
| Infecting bacteria | Treatment | IL-8, ng/ml | IL-1β, ng/ml |
|---|---|---|---|
| None | Medium | 13.26 ± 3 | 0 |
| S. sonnei | Medium | 116.9 ± 8.8 | 1.51 ± 0.01 |
| S. sonnei | S. sonnei preimmune | 114.3 ± 14.9 | 1.38 ± 0.2 |
| S. sonnei | S. sonnei immune | 18.4 ± 0.6 | 0.01 |
| S. sonnei | S. flexneri 2a immune | 107.5 ± 3.6 | 1.58 ± 0.06 |
| S. flexneri 2a | Medium | 119.9 ± 17.3 | 1.54 ± 0.02 |
| S. flexneri 2a | S. flexneri 2a preimmune | 118.53 ± 7.46 | 1.56 ± 0.03 |
| S. flexneri 2a | S. flexneri 2a immune | 17.62 ± 1.1 | 0 |
| S. flexneri 2a | S. sonnei immune | 128.5 ± 1.3 | 1.56 ± 0.02 |
Bacteria were pretreated with preimmune or immune sera to S. sonnei (volunteer 2) or S. flexneri 2a (volunteer 6) and then added to Caca-2 cells as described. The supernatants were assayed by ELISA for IL-8 and IL-1β concentrations. The results are expressed as the means and SEs of three experiments performed in triplicate (IL-8 and IL-1β preimmune vs. immune, P < 0.001).