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. 2007 Feb 6;104(7):2301–2306. doi: 10.1073/pnas.0608382104

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© 2007 by The National Academy of Sciences of the USA

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Fig. 3. — Erythroid progenitor cells are decreased in Hif-2α^Δ/Δ bone marrow but increased in the spleen. (A) Hif-2α^Δ/Δ bone marrow progenitor cells form fewer erythroid colonies in methylcellulose than Hif-2α^fl/Δ cells do (n = 4). (B) In contrast, Hif-2α^Δ/Δ splenic progenitor cells have a higher potential to form erythroid colonies than cells from Hif-2α^fl/Δ spleens (n = 4). (C) There is little difference in the number of nonerythroid colonies formed by Hif-2α^fl/Δ and Hif-2α^Δ/Δ bone marrow cells (n = 4). (D) The percentage of Ter119^high/CD71^high erythroid progenitor cells is reduced in Hif-2α^Δ/Δ bone marrow, but increased in Hif-2α^Δ/Δ spleen (n = 3). (E) Representative FACS blots for Hif-2α^fl/Δ and Hif-2α^Δ/Δ bone marrow and spleen stained for Ter119 and CD71.