Fig. 4.

Activation of AWB neurons, but not of ASH neurons, causes lawn avoidance. (A) 2-Nonanone induces lim-4-dependent avoidance of an OP50 lawn. Asterisks denote values different from N2 by Dunnett test: ∗, P ≤ 0.05 (n ≥ 4 independent trials for each genotype and ≥14 animals in each trial). (B) The osmotic repellent glycerol does not induce avoidance of an OP50 lawn. The repellents SDS and CuCl₂ induce avoidance of an OP50 lawn, but the responses are largely osm-9-independent, and therefore it is likely that they are ASH-independent. Asterisks denote values different from N2: ∗∗, P ≤ 0.01 by unpaired t test (n ≥ 6 independent trials for each condition and ≥18 animals in each trial). (C) Avoidance of purified surfactants added to a 10-μg OP50 lawn (n ≥ 15 independent trials for each chemical and ≥19 animals in each trial). (D) Avoidance of purified surfactants in the acute drop test assay (n ≥ 5 independent trials for each condition and ≥30 animals in each trial). Error bars indicate SEM.