Fig. 1.

MCP-1 expression is up-regulated after retinal detachment (RD) in mice. (A–D) Up-regulation of MCP-1 after RD. (A) Quantitative real-time PCR data for MCP-1 mRNA 72 h after RD (n = 6). (B) ELISA to detect MCP-1 protein 72 h after RD (n = 6). ∗∗, P < 0.01. (C–E) Immunoreactivity of MCP-1 in control retina (C) or after RD in WT mice (D) or in MCP-1^−/− mice (E). (F–H) MCP-1 localization in Müller glia. Double IHC was carried out with antibodies against MCP-1 (F) and glutamine synthetase, a marker for Müller cells (G). Arrows indicate colocalization. (H) Overlay. (Scale bar: 50 μm.)