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. 2007 Feb 5;104(7):2283–2288. doi: 10.1073/pnas.0610877104

Fig. 2.

Fig. 2.

recCaspase-3 activity in HeLa cells. (A–C) Induction of recCaspase-3 activity with 1 μg/ml doxycycline. (A–C Left) Fluorescent micrographs. (A–C Right) DIC photomicrograph of the same field. Without doxycycline (A), the caspase3-sensor EYFP is found throughout cells; after induction (B and C), EYFP fluorescence localizes to the nucleus. (C) Dying cells (arrows) exhibiting the characteristic apoptotic morphology. (D) recCaspase-3, but not caspase-3, subunits are active in HeLa cells. Percentages of nuclear-localized GFP cells out of the total GFP-positive cells are given. Observations from three independent experiments were used to calculate mean and SEM.