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. 2007 Feb 5;104(7):2343–2348. doi: 10.1073/pnas.0610504104

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© 2007 by The National Academy of Sciences of the USA

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Fig. 4. — RT-PCR analysis of KB208E9 and rp11-442e11 mRNA in urine of prostate cancer patients. RNA was isolated from individual urine specimens, and RT-PCR was performed with sequence-specific primers for KB208E9, rp11-442e11, and GAPDH. PCRs were performed for 30 cycles. Numbers below represent the ratio of KB208E9 to rp11-442e11, based on densitometry. GAPDH is shown as an indicator of RNA in each sample. (A) The level of KB208E9 (probe a) and rp11-442e11 (probe b) in the urine RNA of a healthy man (HM1) and nine prostate cancer patients (A–I). (B) The level of KB208E9 (probe a) and rp11-442e11 (probe b) in urine RNA of nine healthy men (HM1-HM9). (C) The mean ratio of KB208E9 to rp11-442e11 in healthy men (0.66 ± 0.12; n = 9) vs. prostate cancer patients (4.04 ± 1.67; n = 9). ∗, Approximate value; a more reliable value could not be obtained because of low rp11-442e11 levels in the sample.