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. 2007 Feb 7;104(7):2223–2228. doi: 10.1073/pnas.0602222104

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© 2007 by The National Academy of Sciences of the USA

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Fig. 2. — The cytosolic domain of retrolinkin dissociates with vesicle membrane. (A) Expression of the N-terminal region of retrolinkin (amino acids 173–406) fused to GFP (retrolinkin-cyto-GFP) in transfected COS7 cells. RTLN, endogenous retrolinkin; retrolinkin-cyto-GFP, cytosolic domain of retrolinkin epitop-tagged with GFP. The blot was probed first with antiretrolinkin (lanes 1–3) and then with anti-GFP (lanes 4–6). (B and C) Unlike full-length retrolinkin (B), the cytosolic domain of retrolinkin without membrane docking sites (retrolinkin-cyto-GFP; C) does not display vesicular structures. Arrowheads in B point to the vesicle structures, and arrowheads in C indicate diffuse distributions. (D) COS7 cells transfected with pEGFP-N2 as a control. (Scale bar: 18 μm.)