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. 2007 Feb 7;104(7):2223–2228. doi: 10.1073/pnas.0602222104

Fig. 4.

Fig. 4.

Full-length retrolinkin function is required for retrograde axonal transport. (A–F) Vesicle fusion and accumulation caused in BPAG1 knockout mouse. EM of longitudinal sections of spinal cord dorsal column reveals fusion and accumulation of vesicles, multivesicular bodies, and other organelles in BPAG1 null (B–F), but not in WT (A). (Scale bar: 1 μm.) (G and H) BPAG1n4–retrolinkin interaction is required for retrograde transport in sensory axons. Transport assays of transferrin-labeled vesicles in cultured DRG neurons overexpressing GFP (G) or retrolinkin-cyto-GFP (H). Shown are montages of vesicle transport over time in a segment of neurite. Velocities of vesicle transport are indicated at the bottom. (Scale bar: 5 μm.) (I) Quantitative measurement of transport assays for MitoTracker-labeled mitochondria and transferrin-labeled vesicles in DRG neurons overexpressing GFP or retrolinkin-cyto-GFP.