Table 1.

Symmetrical self-renewal in culture of Lnk −/− CD34⁻ KSL cells but not WT CD34⁻ KSL cells

Single CD34− KSL cells	Daughter cells		No. of pairs (%)	% Chimerism
	One	The other
WT	+	+	0/28 (0)
WT	+	−	3/28 (11)	0.6, 0.6, 0.6
Lnk−/−	+	+	3/20 (15)	(2.0; 5.9), (50.9; 72.9), (42.9; 81.4)
Lnk−/−	+	−	3/20 (15)	0.7, 2.4, 23.0

Single WT or Lnk−/− CD34⁻KSL cells were cultured with SCF and TPO. After a single cell gave rise to paired-daughter cells, each daughter cell was individually transplanted (SI Fig. 6C). Twelve weeks after transplantation reconstitution was observed in 4 of 25 mice or 3 of 16 mice that, respectively, had received single WT or Lnk−/− CD34⁻KSL cells at day 0. Successful reconstitution is shown as +, and undetectable reconstitution is shown as −, for each transplanted daughter cell.