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. 2007 Jan 31;104(7):2035–2042. doi: 10.1073/pnas.0611125104

Fig. 1.

Fig. 1.

Identification of a highly stable dicistronic pre-mRNA in the cytoplasm. (A) Northern blot of TcUBP RNA. Total RNA from the insect stage of the parasite was electrophoresed in an agarose gel and hybridized with the probes indicated below the images. (B) Schematic structure of the polycistronic gene cluster that contains TcUBP2 and TcUBP1 genes, the pre-mRNA unit, and the TcUBP1 and TcUBP2 monocistronic RNAs. (C) TcUBP RNA is localized in the cytoplasm. Total RNA (T), poly(A)+ RNA (pA+), and cytoplasmic (C) and nuclear (N) RNA fractions from insect-stage parasites were separated in an agarose gel and hybridized with the probes indicated below the images (schemes are not to scale). (D) TcUBP mRNA half-life was determined by ActD treatment. Insect-stage parasites were incubated with the drug, and total RNA was extracted at 0, 1, 2, 4, 6, and 8 h of treatment. Samples were separated on agarose gels and hybridized with the TcUBP probe. Northern blot signals were quantified by using Kodak (Rochester, NY) Image Software and plotted. The relationships between the percentage of mRNA remaining vs. time (hours in ActD) are shown. Molecular weight markers are shown on the right. The systematic gene name for TcUBP1 is Tc00.1047053507093.220 and for TcUBP2, Tc00.1047053507093.229.