Table 1.
Protein-kinase phosphopeptides from S. cerevisiae
| Protein* | Copies per cell† | Peptide sequence | E value‡ |
|---|---|---|---|
| YAL017W | 1,000 | EFAGRRSpSLPRTSASANHLMNRNK | 1.E-02 |
| KSpSSRISTGTLK | 1.E-305 | ||
| RGNQPVSTFLRpTPEK | 1.E-07 | ||
| YBR059C | 3,000 | QSSDPTISEQpSPRLNTQSLPQRQK | 1.E-03 |
| YDL101C | 3,500 | REHpSGDVTDpSpSFK | 1.E-03 |
| REHpSGDVTDpSSFK | 1.E-06 | ||
| REHpSGDVTDSpSFK | 1.E-07 | ||
| REHpSGDVTDSSFK | 1.E-306 | ||
| YDR122W | 300 | FGNIFRKLpSQRRK | 1.E-05 |
| FGNIFRKLpSQRRKK | 1.E-305 | ||
| IRNQQQRQpSHENIEK | 1.E-305 | ||
| pSVGHARREpSLK | 1.E-06 | ||
| pSVGHARRESLK | 1.E-04 | ||
| YDR283C | 300 | pSIQNVPRRRNFVK | 1.E-04 |
| YDR490C | ND | GNRpSLTEADHALLSK | 1.E-02 |
| YDR507C | 700 | KRQpSIpSSVSVSPpSK | 1.E-02 |
| KVSTpTPQRRRNRESLISVTpSpSRK | 1.E-06 | ||
| KVSTpTPQRRRNRESLISVTSpSRK | 1.E-03 | ||
| KVSTpTPQRRRNRESLISVTSSRK | 2.E-05 | ||
| QNpSQEGDQAHPK | 1.E-305 | ||
| SRHFpSEpSNK | 1.E-06 | ||
| YER129W | 800 | QRTHERpSRSLTVAELNEEK | 1.E-07 |
| YHL007C | 300 | pSKTSPIISTAHTPQQVAQSPK | 1.E-05 |
| SKTpSPIISTAHTPQQVAQpSPK | 1.E-04 | ||
| pSSTDIRRATPVSTPVISK | 1.E-03 | ||
| QTHAPTTPNRpTSPNRpSSISRNATLK | 1.E-02 | ||
| QTHAPTTPNRpTSPNRSpSISRNATLK | 1.E-05 | ||
| YHR082C | 1,300 | ELEQERRLpSMEQK | 1.E-03 |
| YRRPpSSSSYTGK | 1.E-04 | ||
| YHR205W | 4,000 | KKPLYTHRpSSSQLDQLNSCSSVTDPSK | 1.E-304 |
| YIL095W | 1,300 | SRQNTGDpSIRSAFGK | 1.E-03 |
| YJL095W | 100 | REAPKPPANTpSPQRTLSTSK | 1.E-07 |
| YJL128C | 2,200 | RSApSVGSNQSEQDK | 1.E-09 |
| RTSpSTSSHYNNINADLHARVK | 1.E-02 | ||
| YJL141C | ND | FRRApSLNpSK | 1.E-03 |
| FRRApSLNSK | 1.E-09 | ||
| YJL165C | ND | RARpSLpSESIK | 1.E-04 |
| RARpSLSESIK | 1.E-305 | ||
| GDEKLpSRHpTSLK | 1.E-04 | ||
| YJR059W | 1,400 | STpSTNDFSENSLDAPHDQEVIHTSNPFLK | 1.E-08 |
| RPTSPSISGSGSGGNSPSSSAGARQRpSASLHRRK | 1.E-05 | ||
| RPpTSPSISGSGSGGNSPSSSAGARQRSASLHRRK | 1.E-04 | ||
| STSpTNDFSENSLDAPHDQEVIHTSNPFLK | 1.E-08 | ||
| YKL101W | <50 | LRLpSPENPSNTHMQK | 1.E-305 |
| RRPpSEEpSVNPK | 1.E-305 | ||
| RRPpSEESVNPK | 1.E-06 | ||
| SPpSRYSLSRRAIHASPpSTK | 1.E-305 | ||
| SPpSRYSLSRRAIHASPSTK | 1.E-05 | ||
| YKL126W | 3,000 | EEQQNNQATAGEHDASITRSpSLDRK | 1.E-03 |
| HSGFFHSSKKEEQQNNQATAGEHDASITRSpSLDRK | 1.E-304 | ||
| KEEQQNNQATAGEHDASITRpSSLDRK | 1.E-07 | ||
| YKL139W | 100 | RPLFGKRpSPNPQSLARPPPPK | 1.E-03 |
| YKL166C | 1,600 | MYVDPMNNNEIRKLpSITAK | 1.E-305 |
| YKL168C | 1,000 | RQQPVTRRVRpSFSESFK | 1.E-305 |
| YLR096W | ND | LTIPEQAHTSPpTSRK | 1.E-08 |
| QHSLPpSPKNESEILERQK | 1.E-07 | ||
| YLR248W | 1,800 | DVSQITSpSPKK | 1.E-305 |
| YMR001C | 1,500 | LEEYHQNRPFLPHSLpSPGGTK | 1.E-06 |
| YMR216C | 2,400 | RLQRHVSRSpSDITANDSSDEK | 1.E-04 |
| YMR291W | 10,000 | TLpSRQGpSSTpSVKK | 1.E-06 |
| KNpSpTFVLDPKPPK | 1.E-04 | ||
| TLpSRQGpSSTSVKK | 1.E-08 | ||
| DTPNFSFHPTIRRVSpSTASMHTLRSPSK | 1.E-06 | ||
| YNL020C | 1,500 | TRpSLGSYSTRGNIK | 1.E-09 |
| YOL016C | 7,500 | TLHDDREpSK | 1.E-305 |
| YOL100W | <50 | ATPQRSTpSNRNVGDLLLEK | 1.E-03 |
| HNpSFpSESINpSAK | 1.E-02 | ||
| VIERRTpSSSGRAIPK | 1.E-05 | ||
| YOL128C | ND | TYFCpSRFYRAPELLLNSK | 1.E-305 |
| YOR267C | 300 | KLpSMSQLRSKK | 1.E-03 |
| QTHpSMAELK | 1.E-09 | ||
| YPL150W | <50 | ISSQRAYSHSIAGpSPRK | 1.E-06 |
*Standard gene names from the Saccharomyces genome database, www.yeastgenome.org.
†Protein expression levels obtained from genome-wide protein affinity purification experiments (4), ND means no detected expression.
‡Expectation scores assigned by Open Mass Spectrometry Search Algorithm. An E value <0.05 is statistically significant. With current search parameters, spectra assigned scores higher than 1.E-04 should be subjected to manual interpretation.