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. 2000 Dec 12;97(26):14400–14405. doi: 10.1073/pnas.260501497

Figure 2.

Figure 2

Colocalization of 14-3-3 and HDACs. (A) Cos cells were transfected with expression vectors for FLAG-tagged derivatives of HDAC4 or -5, or a Myc-tagged version of 14-3-3ɛ (1 μg each). The subcellular distribution of ectopic proteins was determined by indirect immunofluorescence by using primary antibodies against the epitope tags and fluorescein (HDAC)- or rhodamine (14-3-3)-conjugated secondary antibodies. (B and C) Cos cells were cotransfected with expression plasmids (0.5 μg each) for FLAG-tagged HDAC4 (B) or HDAC5 (C) and Myc-tagged 14-3-3 in the absence (a, c) or presence (b, d) of an expression vector for constitutively active CaMKI (0.5 μg). Cells were costained with an anti-FLAG monoclonal antibody and polyclonal anti-Myc antibody, followed by fluorescein (HDAC)- or rhodamine (14-3-3)-conjugated secondary antibodies. (D) Subcellular distribution of ectopic14-3-3ɛ in the absence or presence of activated CaMKI.