TABLE 2.
Effect of manipulation of components of the Wsp pathway on colony morphology of ancestral SM and derived LSWS genotypes
| Genotype and colony morphology of initial genotype | Genetic manipulationa | Effect on colony morphology |
|---|---|---|
| SM | ΔwspC | SM |
| SM | ΔwspF | WSb |
| SM | ΔwspR | SM |
| SM | pVSP61-wspC | WSb |
| SM | pVSP61-wspF | SM |
| SM | pVSP61-wspR | WSb |
| SM | pVSP61-wspE | SM |
| LSWS | ΔwspC | SMb |
| LSWS | ΔwspF | WS |
| LSWS | ΔwspR | SMb |
| LSWS | pVSP61-wspC | WS |
| LSWS | pVSP61-wspF | SMb |
| LSWS | pVSP61-wspR | WS |
| LSWS | pVSP61-wspE | SMb |
a
Manipulation involved either deletion of a gene (e.g., ΔwspC) or overexpression of the gene on plasmid pVSP61 (e.g., pVSP61-wspC).
b
Conversion of phenotype.