TABLE 3.

wspF mutations from independent WS genotypes

WS genotypea	Nucleotide change	Nature of mutation	Amino acid change	Predicted effect on function and groupb
LSWS	A901C	Transition	S301R	Reduced activity (2); mutation close to active site
WSA	T14G	Transversion	I5S	Reduced activity (2); mutation close to active site
WSB	Δ620-674	Deletion	P206Δ(8)c	No activity (3); C-terminal third of protein lost
WSC	G823T	Transversion	G275C	Reduced activity (2); mutation close to active site
WSE	G658T	Transversion	V220L	Reduced activity (2)
WSF	C821T	Transversion	T274I	Reduced activity (2); mutation close to active site
WSG	C556T	Transversion	H186Y	No activity (3); catalytic residue altered
WSJ	Δ865-868	Deletion	R288Δ(3)c	Reduced activity (2); most of protein remains
WSL	G482A	Transition	G161D	Reduced activity (2)
WSN	A901C	Transition	S301R	Reduced activity (2); same mutation as in LSWS
WSO	Δ235-249	Deletion	V79Δ(6)c	No activity (3); C-terminal two-thirds lost
WSU	Δ823-824	Deletion	T274Δ(13)c	No activity (3); Asp286 active site residue is lost
WSW	Δ149	Deletion	L49Δ(1)c	No activity (3); most of protein lost
WSY	Δ166-180	Deletion (in frame)	Δ(L51-I55)	Reduced activity (2); small in-frame deletion of five residues

^aNo wspF (wspC or wspR) mutation was detected in WS_D, WS_H, WS_I, WS_K, WS_M, WS_P, WS_Q, WS_R, WS_S, WS_T, WS_V, WS_X, and WS_Z.

^bDefects caused by each mutation were scored on the basis of the likely magnitude of the effect (see text): 1, wild-type wspF (13 isolates); 2, reduced activity (9 isolates); 3, no activity (5 isolates).

^cP206Δ(8) indicates a frameshift; the number of new residues before a stop codon is reached is in parentheses.