TABLE 1.

Statistical analysis of qRT–PCR data

Linea	Tissue	n (control, sample)b	Average fold changec	Direction	Significanced	Target	Treatment
FC606	Leaf	6, 5	1.113	Down	P ≥ 0.7236	BvFL1_v2/v3	45 days vernalization
FC606	Leaf	6, 6	2.909	Down	P ≤ 0.0377	BvFL1_v2/v3	90 days vernalization
FC606	Leaf	6, 5	2.646	Up	P ≤ 0.0064	BvFL1_v2/v3	17 days postvernalization
FC606	Shoot apex	2, 2	1.222	Down	P = 0.3323	BvFL1_v2/v3	77 days vernalization
SLC003	Leaf	4, 4	2.948	Down	P ≤ 0.0199	BvFL1_v2/v3	90 days vernalization
SLC003 vs. FC606	Shoot apex	2, 2	1.532	SLC003 > FC606	P = 0.5027	BvFL1_v2/v3	No vernalization
FC606	Leaf	4, 4	1.732	Down	P ≥ 0.1826	BvFL1_v1/v4	90 days vernalization
FC606	Shoot apex	2, 2	1.023	Down	P = 0.8347	BvFL1_v1/v4	77 days vernalization
SLC003	Leaf	4, 4	1.961	Down	P ≤ 0.0025	BvFL1_v1/v4	90 days vernalization
SLC003 vs. FC606	Shoot apex	2, 2	1.089	SLC003 > FC606	P = 0.8347	BvFL1_v1/v4	No vernalization
FC606	Leaf	4, 4	1.101	Up	P ≥ 0.7356	BvFL1_v1–v4	90 days vernalization
FC606	Leaf	6, 4	1.297	Up	P ≥ 0.4685	BvFL1_v1–v4	No vernalization
SLC003 vs. FC606	Leaf	4, 6	1.478	SLC003 > FC606	P ≥ 0.4443	BvFL1_v1–v4	Two- to seven-leaf stage

^aFC606, biennial; SLC003, annual.

^bSLC003 used as control for comparisons between lines.

^cAverage magnitude of change, calculated from the two independent qRT–PCR setups.

^dFor statistically significant results (P < 0.05), the larger of the P-values calculated from replicated qRT–PCR setups is reported. For nonsignificant results, the smallest P-value is shown.