TABLE 2.
Plasmids used in this study
| Plasmid | Description | Source |
|---|---|---|
| pABP1-SH3 | Codons 535-592 of ABP1 were PCR amplified and subcloned into pET-21d+ (Novagen, Madison, WI) to give ABP1 SH3 with a C-terminal 6-His tag under the control of the T7 promoter. | Rath and Davidson (2000) |
| pABP1-SH3-N53A | pABP1-SH3 derivative containing ABP1 SH3 with the N53A substitution | This study |
| pABP1-SH3-Y54A | pABP1-SH3 derivative containing ABP1 SH3 with the Y54A substitution | This study |
| pABP1-SH3-Y54V | pABP1-SH3 derivative containing ABP1 SH3 with the Y54V substitution | This study |
| pABP1-SH3-Y54P | pABP1-SH3 derivative containing ABP1 SH3 with the Y54P substitution | This study |
| pGST-SRV2-CTR | Contains C-terminal SRV2 coding sequence (codons 253–526), including the K325A and K326A substitutions, fused to the C-terminus of GST; allows for expression of GST-Srv2-CTR in bacteria. | Mattila et al. (2004) |
| pGAL-ARK1-GFP | Contains ARK1 coding sequence, including the kinase dead K56A substitution, fused to the N terminus of GFP (wild type); allows for expression of Ark1-GFP under the inducible GAL1,10 promoter. | Cope et al. (1999) |
| p316ΔSalI-ABP1 | A 3.5-kb fragment containing the ABP1 ORF plus 1.5 kb upstream and 180 bp downstream was subcloned into p316 ΔSalI | This study |
| p316ΔSalI-ABP1-ΔSH3 | p316ΔSalI-ABP1 derivative containing ABP1 with the SH3 domain deletion | This study |
| p316ΔSalI-ABP1-SH3-N53A | p316ΔSalI-ABP1 derivative containing ABP1 SH3 with the N53A substitution | This study |
| p316ΔSalI-ABP1-SH3-Y54A | p316ΔSalI-ABP1 derivative containing ABP1 SH3 with the Y54A substitution | This study |
| p316ΔSalI-ABP1-SH3-Y54V | p316ΔSalI-ABP1 derivative containing ABP1 SH3 with the Y54V substitution | This study |
| p316ΔSalI-ABP1-SH3-Y54P | p316ΔSalI-ABP1 derivative containing ABP1 SH3 with the Y54P substitution | This study |
| p315-ABP1 | A 3.5-kb fragment containing the ABP1 ORF plus 1.5 kb upstream and 180 bp downstream was subcloned into pRS315. | This study |
| p315-ABP1-ΔSH3 | p315-ABP1 derivative containing ABP1 with the SH3 domain deletion | This study |
| p315-ABP1-SH3-Y54A | p315-ABP1 derivative containing ABP1 SH3 with the Y54A substitution | This study |