TABLE 3.
In vitro binding data for Abp1p SH3 domain mutants
| Abp1p SH3 protein | Peptide binding [Kd (μm)]a
|
|||
|---|---|---|---|---|
| Ark1 | Srv2 | Scp1 | Tm (°C)b | |
| Wild type | 0.27 ± 0.02 | 4.43 ± 0.4 | 1 ± 0.05 | 57 |
| N53A | 1.12 ± 0.06 | 5.56 ± 0.47 | NT | 62 |
| Y54V | 5.67 ± 0.3 | 16.1 ± 1.73 | 6.7 ± 2.3 | 55 |
| Y54A | 15.3 ± 1.2 | >50c | 7.2 ± 1.9 | 57 |
| Y54P | >50c | NT | NT | 30 |
NT, not tested.
a
Determined by isothermal titration calorimetry.
b
Determined by in vitro thermal unfolding experiments.
c
Binding was not detected; an interaction with a Kd of < ∼50 μm is detectable in this assay.