TABLE 4.
Summary of growth data for prk1Δ, sla1Δ, sac6Δ, and sla2 mutants that express different Abp1p SH3 domain derivatives
| Abp1p SH3 domain mutant |
prk1Δ
|
sla1Δ
|
sac6Δ
|
sla2Δ
|
sla2-Δcoil1
|
|||||
|---|---|---|---|---|---|---|---|---|---|---|
| SCa | SC + cafb | SC | SC + caf | SC | SC + caf | SC | SC + caf | SC | SC + caf | |
| Wild type | ++ | ++ | ++ | ++ | ++ | ++ | + | NTc | ++ | ++ |
| N53A | ++ | ++ | ++ | ++ | ++ | ++ | + | NT | ++ | ++ |
| Y54V | ++ | ++ | ++ | ++ | ++ | +/− | +/− | NT | ++ | +/− |
| Y54A | ++ | + | ++ | + | +/− | − | − | NT | − | − |
| Y54P | +/− | − | +/− | − | − | − | − | NT | − | − |
| ΔSH3 | +/− | − | +/− | − | − | − | − | NT | − | − |
++, wild-type growth; +, slow; +/−, very slow; −, approximately no growth.
a
SC, synthetic complete agar plates, incubated for 3 days at 30° (sla1Δ, sac6Δ, sla2Δ) or 37° (prk1Δ, sla2-Δcoil1).
b
caf, 3 mM caffeine.
c
NT, not tested; sla2Δ cells do not grow in this condition.