TABLE 2.
Spontaneous pyrE2 mutations
| Mutation | No.a | Sequence contextb |
|---|---|---|
| T185 → G | 1 | 179ACAAACTGGCCGG191 (Leu to Arg) |
| C206 → A | 1 | 200TCGGCGCGGTCCC212 (Ala to Glu) |
| −15 bp | 2 | 300CGAGGGCG[7 bp]CGAGGGCG322 |
| +51 bp | 5 | 378CGTGGA[45 bp]CGTGGA434 |
| −C417 | 1 | 411CGACCGCGTCGTC423 |
| −3 bpc | 3 | 417CGTCGTCGTCGTCGT431 |
| +3 bpc | 1 | 417CGTCGTCGTCGTCGT431 |
| −51 bp | 3 | 457CTGCTGGCCGAC[39 bp]CTGCTGGCCGAC519 |
a
Number of independent isolates containing this mutation.
b
Superscripts indicate the position of the first and last posted nucleotide, where A in the initiating ATG is 1 (Bitan-Banin et al. 2003). For the multiple-base indels, the direct repeats defining the ends are underlined. The numbers in brackets indicate the numbers of bases deleted or duplicated, to which is added the number in one copy of the flanking sequence. For the three single-base mutations, six nucleotides are shown before and after the underlined wild-type base.
c
Due to the repetitive nature of this region, the original event could have added or removed CGT, GTC, or TCG.